yhfP [2018-09-26 09:21:59]

similar to predicted oxidoreductase, Zn-dependent and NAD(P)-binding

locus: BSU10320

pI: 4.72

mw: 34.60 kDa

protein length: 330 aa

gene length: 990 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 1,106,524 → 1,107,516

The protein

Protein family

• Quinone oxidoreductase subfamily (according to Swiss-Prot)

Structure

• [PDB|1TT7] (99% identity)
• [PDB|1Y9E] (with NAD), [PDB|1TT7]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|DCA3A7605CA19FAF61BDB75421BD41F11CB638E5|yhfO]-[gene|FE034FDCEAD4F4E91FD0147E16685632069E3316|yhfP]-[gene|1469148C83B1704E721652203EA31F0E2C33FDC6|yhfQ]
  description: [Pubmed|22383849]

• genes: [gene|FE034FDCEAD4F4E91FD0147E16685632069E3316|yhfP]-[gene|1469148C83B1704E721652203EA31F0E2C33FDC6|yhfQ]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A715 (yhfP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/715 NBRP B. subtilis, Japan]
• BKE10320 (Δ[gene|FE034FDCEAD4F4E91FD0147E16685632069E3316|yhfP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCGGCACACTCCTTTTC, downstream forward: _UP4_TAACAGGATCAGCTTGCAGA
• BKK10320 (Δ[gene|FE034FDCEAD4F4E91FD0147E16685632069E3316|yhfP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCGGCACACTCCTTTTC, downstream forward: _UP4_TAACAGGATCAGCTTGCAGA